C.B.S. Scientific TTGE-2401 - Manual (Page 2)

ClearPAGE Precast Gel Running Instructions 7-1-14 2
GENERAL INSTRUCTIONS
1) Use ONLY ClearPAGE buffers with ClearPAGE TEO gels. Ordering
information for these reagents is on pages 12-14. Alternatively, you can
make your own by following the buffer formulations on pages 9-10.
2) The running and loading buffers must be consistent with the type of gel being
used (SDS PAGE or DNA/Native)
3) Do not substitute or mix reagents with those from other sources.
4) Use only ultrapure water for washing and dilution purposes.
5) These products should only be used by qualified personnel who have had
laboratory safety instruction.
6) The complete instructions should be read and understood before attempting to use
the product.
7) Products should be used before the “Best by” date on the product label.
8) Wear gloves at all times when handling gels, buffers, etc.
9) Before running first gel in the DCX-700, QNC-700 or QNX-700 please read
the Dual Cool/Quadra Instruction manual for important safety and general use
instructions. To open DualCool/Quadra, push thumbs down on white/clear posts
while pulling lid up with fingers as shown on right. DO NOT PULL UP ON
LEADS!
RUNNING BUFFER PREPARATION
Type of Gel Running Buffer Cat. #’s Dilution factor
ClearPAGE Neutral pH -TEO SDS-Non-Reducing: (cat. # FB50053 or FB50500)
SDS-Reducing: (cat. # FB60053 or FB60500)
Turbo SDS: (cat.# CB13500)
20X
20X
20X
DNA/Native DNA/Native: (cat. # GB61053 or GB61500) 20X
ClearPAGE™ SDS or ClearPAGE DNA/NATIVE Running Buffers MUST be
used to run these gels. To make 1 liter of 1x running buffer (for example: dilute
50ml 20x Running Buffer with 950ml ultrapure water or dilute 100mls of 10X
buffer with 900mls of ultrapure water) for a run. For the ClearPAGE Running
Buffer bottle included in the Sampler Kits, dilute by adding its contents to 950ml
ultrapure water. Remember, to save time and money you can use reconstituted
ClearPAGE running buffer for the bottom reservoir by following instructions
below:
RECYCLING BUFFER INSTRUCTIONS: The buffer may be reused on the
anode side, but fresh buffer is ALWAYS required in the cathode chamber. When
reusing buffer make sure used buffer from upper core gets recycled as well. This
can be accomplished by removing the gel cassettes while the core is still in the
unit and letting the buffer drain into the lower chamber. Pour this reconstituted
buffer into a separate container for reuse in the anode chamber of your next gel
run! This buffer can be used time and time again. For fresh upper core buffer
dilute 10 ml of ClearPAGE™ Running Buffer (20x) to 200 ml with ultrapure
water.
ClearPAGE ™ Gel Running Instructions
FOR USE WITH: C.B.S. Scientific’s Combo 2-place Dual Cool System (DCX-700) and
Combo 4-place Quadra Systems (QNC-700 & QNX-700)