Thermo Fisher Scientific Ion Selective Electrodes Copper - Manual (Page 5)

Instruction Manual Copper Electrode
5
may be prepared by adding 24.16 grams of reagent-grade
Cu(NO
3
)
2
∃ 3H
2
O to a 1 liter volumetric flask about
half full of distilled water. Swirl the flask to
dissolve the solid. Fill flask to the mark with
distilled water, cap the flask, and upend it several
times to mix the solution thoroughly.
4. Cupric Nitrate Standard Solution, 1000 ppm Cu
+2
. This
solution may be prepared by adding 3.80 grams of
reagent-grade Cu(NO
3
)
2
∃ 3H
2
O to a 1 liter volumetric
flask about half full of distilled water. Swirl the
flask to dissolve the solid. Fill the flask to the
mark with distilled water, cap the flask, and upend it
several times to mix the solution thoroughly.
5. EDTA Titrant (1M) for Cupric Titrations. To prepare
this solution from your own laboratory stock, add 37.2
grams of reagent-grade Na
2
EDTA
∃ 2H
2
O to a 100 ml
volumetric flask about three-fourths full of distilled
water. Swirl the flask to dissolve the solid. Add
distilled water to the mark, cap the flask, and upend
it several times to thoroughly mix the solution.
GENERAL PREPARATION
Electrode Preparation
Remove the rubber cap(s) covering the electrode tip(s) and the
rubber insert covering the filling hole of the cupric combination
ion electrode or the reference electrode. Fill the reference
electrode or the combination electrode with the filling solution
shipped with the electrode to a level just below the fill hole.
No preparation is required with a sealed reference electrode.
Connect the electrode(s) to the proper terminal(s) as recommended
by the meter manufacturer.
Electrode Slope Check (with standard pH/mV meter)
(check electrodes each day)
1. To a 150 ml beaker, add 100 ml of distilled water and 2
ml of ISA. Place the beaker on the magnetic stirrer
and begin stirring at a constant rate. After assuring
that the meter is in the mV mode, lower the electrode
tip(s) into the solution.
2. Using a pipette, add 1 ml of either the 0.1M or 1000
ppm cupric standard to the beaker. When the reading is
stable, record the millivolt reading.