Thermo Fisher Scientific Ion Selective Electrodes Copper - Manual (Page 10)

Instruction Manual Copper Electrode
10
and blotting dry, lower the electrode tip(s) into the
solution. When the reading has stabilized, record the
reading. Determine the concentration directly from the
calibration curve.
11. The calibration should be checked every two hours.
Assuming no change in ambient temperature, immerse the
electrode tip(s) in the midrange standard. After the
reading has stabilized, compare it to the original
reading recorded in step 5 above. If the reading
differs by more than
∀0.5 mV, or the temperature has
changed from ambient, recalibrate the electrode.
Direct Measurement of Cupric Ion (using an ion meter)
1. By serial dilution of the 0.1M or 1000 ppm cupric
standard, prepare two cupric standards whose
concentration is near the expected sample
concentration. Measure out 100 ml of each standard into
individual 150 ml beakers and add 2 ml of ISA to each.
2. Place the more dilute solution on the magnetic stirrer
and begin stirring at a constant rate. Assure that the
meter is in the concentration mode.
3. Lower the electrode tip(s) into the solution.
4. Adjust the meter to the concentration of the cupric
standard and fix the value in the memory according to
the meter manufacturer's instructions after
stabilization of the reading.
5. Rinse the electrode(s) with distilled water and blot
dry.
6. Place the more concentrated solution on the magnetic
stirrer and begin stirring at a constant rate.
7. Lower the electrode tip(s) into the solution
8. Adjust the meter to the concentration of the cupric
standard and fix the value in the memory according to
the meter manufacturer's instructions after
stabilization of the reading.
9. Place 100 ml of the sample and 2 ml of ISA into a 150
ml beaker. Place the beaker on the magnetic stirrer
and begin stirring at a constant rate.
10. Immerse the rinsed, dried electrode tip(s) in the