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Luminex BioPlex XYP, LX200 User Manual (supplied by BioRad) User Manual

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Table of Contents

Page

Section 1 General Information ......................................................................1

1.1

About This Manual ....................................................................................1

1.2 Safety

Information

.....................................................................................1

1.2.1

Electrical Safety Information ...........................................................2

1.2.2 Laser Safety Information ................................................................2
1.2.3 Mechanical Safety Information .......................................................4
1.2.4

Biological Safety Information ..........................................................4

1.2.5 Blue Indicator Light ........................................................................4

Section 2 Introduction ...................................................................................4

2.1

The Bio-Plex Suspension Array System and Multiplexing Technology .......4

2.2

Description of System Components ..........................................................5
2.2.1

Array Reader .................................................................................5

2.2.2

Microplate Platform ........................................................................8

2.2.3

High-Throughput Fluidics (HTF) .....................................................9

2.2.4

Computer and Monitor..................................................................10

2.2.5

Maintenance, Calibration, and Validation Plate ............................10

2.2.6

Bio-Plex Reservoir .......................................................................10

2.3

Recommended Additional Equipment Not Provided ................................11

2.4 Bio-Plex

Assays

......................................................................................11

Section 3 Installation ...................................................................................11

3.1 Unpacking

...............................................................................................11

3.2 System

Location ......................................................................................11

3.3

Microplate Platform Setup .......................................................................12

3.4

Array Reader Setup .................................................................................12

3.5

Connecting the Sheath Fluid and Waste Containers ................................14

3.6

Computer and Monitor Connections ........................................................15

3.7 Software

Installation

................................................................................15

3.7.1

System Software Loading ............................................................15

3.7.2

Communication Ports ..................................................................16

3.8

Installing or Changing the Sample Needle ...............................................16
3.8.1

Installing/Changing the Long Sample Needle ...............................16

3.8.2

Adjusting Sample Needle Height .................................................17

3.9

Initial System Priming ..............................................................................19

3.10

Resetting Instrument Pressure Settings ..................................................20

3.11

High-Throughput Fluidics (HTF) Setup ....................................................20

3.12

Vacuum Manifold Setup ..........................................................................24
3.12.1 System Setup ..............................................................................24
3.12.2 Validation of Vacuum Pressure ....................................................25

3.13

Performing System Validation .................................................................26

Section 4 Care and Maintenance ................................................................27

Section 5 Troubleshooting Guide ...............................................................30

5.1

Troubleshooting Guide for Bio-Plex 200 System .....................................30

5.2

Troubleshooting Guide for Vacuum Manifold ...........................................35

5.3 Technical

Support

...................................................................................36

Section 6 Bio-Plex 200 System Specifications ..........................................36

Section 7 Warranty Statement ....................................................................38

Section 8 Ordering Information – System Accessories ............................39

Section 9 Decontamination Information ....................................................41

Section 10 Legal Notices ..............................................................................42