Hach-Lange LUMISTOX 300 LPV321 User Manual - Manual (Page 36)

LUMIStox 300 Operating Manual Dr. Bruno Lange GmbH
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Page: 36
4.2.2 Practical advice: The geometrical dilution series:
Position the necessary number of LUMIStox measuring cuvettes in row A of the
LUMIStherm. A dilution series of the sample as described in the example below
requires 9 cuvettes for the sample dilutions and 1 cuvette for the control solution.
Pipette into the measuring cuvettes, in sequence:
2% NaCl solution
then prepared sample
Use a pipette to transfer,
proceeding from left to right, 1.5
ml into the following cuvette. Mix
each sample dilution by drawing
it into the pipette two or three
times.
There is no need to change the
pipette tip.
The 2% NaCl control solution always stands in A1. The highest sample con-
centration, in this case the undiluted sample, is on the far right, in this case A10.
1,5 1,5 1,5 1,5 1,5 1,5 1,5 1,5 0,0
1.
1,5 1,5
2.
3.
ml
ml
ml
=
1,5 1,5 1,5 1,5 1,5 1,5 1,5
dilution: 1:
1 2 4 8 16 32 64 128 256
1,5