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Performance – Ocean Optics CUV-TLC-50F User Manual

Page 16

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2: Operation

Note

The CUV-TLC-50F is intended to hold a cuvette with a standard wall thickness of
1.25 mm and total width of 12.5 mm. Cuvettes with unusually thick walls will not
properly fit and may damage the holder. Also, unusually short cuvettes will be difficult to
remove after being pushed all of the way to the bottom of the holder.

3. Place the cuvette and sample in the sample holder.

4. Use the provided optical slits or blanks around the cuvette in a manner that correctly limits

the excitation and emission light.

5. If needed, place the magnetic stir bar in the cuvette and turn the magnetic stirrer knob located

on the front of the temperature control until the desired speed is reached.

6. Place the opaque plastic cover over the cuvette holder.

7. Place the access lid in the hole in the plastic cover.

8. Set the target temperature using the up/down buttons located to the right of the display

window. The normal temperature range is -10 °C to +80 °C, although temperatures from –
40 °C to +85 °C may be used under special circumstances (see

Temperature

in Chapter 3:

Specifications

).

9. Briefly depressing either the up or down button displays the target temperature. Otherwise,

the current temperature will be displayed.

10. To begin controlling the temperature, press the Run/Stop button located to the left of the

display. Pressing the Run/Stop button a second time turns temperature control off.

11. When the sample holder approaches the target temperature, the red light (located on the upper

left corner of the front panel of the temperature controller) flashes slowly.

12. When the sample holder reaches within ± 0.02 °C of the target temperature, the red light will

remain constant.

13. After measurements are completed, depress the run/stop button to stop temperature control,

and turn off power and water sources.

Performance

The graphs in this section show typical performance results for the CUV-TLC-50F under the following
conditions:

Temperature Equilibration
Low Temperatures
Temperature Ramping

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