Olympus IX81-ZDC2 - Manual (Page 11)

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IX81-ZDC2
9
OPERATION
The Class 1 laser beam that is output from the objective is not hazardous (but direct staring for an extended period is still
inhibited), and the laser beam intensity will not increase even in the case of malfunction. However, to prevent an incident,
make sure not to tilt the glass bottom dish so that the reflected laser beam will not be scattered around.
If you use an objective with collection collar, the following adjustment is
necessary.
1. How to perform correction for bottom thickness of the glass-bottom dish:
When the thickness of the bottom of the glass-bottom dish is known,
match the scale reading of the correction collar to the thickness of the
glass bottom.
2. How to find the optimum position based on image resolution and contrast:
· If the thickness of the glass-bottom dish is unknown, the optimum position
for the collection collar can be obtained by judging the image resolution
and contrast. When a satisfactory image is not obtained after focusing,
rotate the correction collar to the left and right, refocus each time and
compare the images at both sides. Then rotate the collar in the direction
yielding a better image, and rotate the collection collar to the left and
right, refocus each time and compare the images. Repeat this cycle until
the position with the optimum image is found.
1. Set the power switches of the IX2-UCB control box and PC to “ I ” (ON).
2. Set the AF port switching lever @ of the AF sensor unit to the pushed-in
position (IN).
} This operation engages the dichroic mirror (DM) in the light path of the
AF sensor unit, making AF detection possible.
If AF detection is not necessary, setting the lever @ to the OUT position
makes it possible to eliminate the small loss of the observation light.
This is also effective in observation with IR light (785 nm or higher
wavelengths).
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3-2 Focus Drift Compensation Procedure
3-1 Operating Precautions
1 Preparation (Fig. 1)
2 Adjusting the Correction Collar
@
Fig. 1