Hoefer PR625 Immunoblot - Manual (Page 9)

•
p7
Recommendation: A 1%
solution of 7X Cleaning
Solution (Flow Laboratories,
50 ml concentrate in 5 liters
of warm water).
Remove and develop the blot
1
Unscrew the ImmunoBlot and separate the two halves.
2
Discard the used sealing pad and wash the membrane
in a tray with 3 to 5 changes of buffer for a total of
10 to 15 minutes.
3
Develop the blot by following instructions from your
Western detection kit (See also Appendix B).
Clean the ImmunoBlot
Clean the unit thoroughly after each use by
performing the following:
1
Rinse the ImmunoBlot unit and washing manifold
under distilled water.
2
Use a non-abrasive, alkaline laboratory cleaning agent
that leaves no residue after rinsing, such as a deter-
gent intended for cleaning tissue culture glassware.
Concentrated cleaning agents should be diluted to
normal strength.
3
Immerse the ImmunoBlot unit and washing manifold
in the cleaning solution and brush gently with a soft
brush taking care not to scratch the interior surfaces.
4
Rinse the ImmunoBlot unit and washing manifold
thoroughly with tap water, followed by distilled water.
To easily rinse the small numbered holes, assemble
the unit and manifold units without a membrane and
sealing pad, and flush with distilled water.
Note: Do not autoclave the
ImmunoBlot unit or washing
manifold.
Do not expose the
ImmunoBlot unit to alcohol or
other organic solvents.