Hoefer PR625 Immunoblot User Manual
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Note 2: Aligning the membrane
For Western blotting experiments it is important that lanes and protein bands
on the membrane align with the channels of the ImmunoBlot unit. This can be
accomplished in 2 steps:
Step 1.
Use the specially designed ImmunoBlot combs at the time of electrophoresis. This ensures
that the lanes on the gel and, subsequently, the lanes and protein bands on the blot, are
aligned with the channels on the ImmunoBlot.
Combs with well spacings that match the lane spacings of the ImmunoBlot units are listed
below. Please note that the number of wells does not always match the number of lanes in
a 1:1 ratio.
Fig A. The 9-well 1.0-mm comb (PR511-9-1.0) aligns with the 45 channels of the ImmunoBlot XL and
can be used to probe 9 samples with up to three probes per sample.
9 well x 45 channel
Sample Wells
Blot Channels
1
2 3 4
5 6
7 8 9
10 11
12 13 14
17 18 19
22 23 24
27 28 29
32 33 34
37 38 39
42 43 44
15 16
20 21
25 26
30 31
35 36
40 41
45
Blot Channels under sample wells
Blot Channels NOT under sample wells