Hoefer PR625 Immunoblot - Manual (Page 8)

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p6
Introduce secondary antibody
and incubate
The secondary antibody incubation may be
performed either in the unit or in a tray.
6
To perform the incubation in the
ImmunoBlot unit:
1
Inject the secondary antibody solution into the chan-
nels carefully with a single or multi-channel pipette.
2
Incubate the unit on a rocking platform with the
channels aligned in the direction of rocking for 30 to
60 minutes at room temperature.
3
Aspirate the secondary antibody solution using a
vacuum source or pipette as described before (page 5,
step 2) and wash the blot with wash buffer for a few
seconds. Washing the blot prevents cross contamina-
tion of channels by different secondary antibodies
across channels when removing the blot from the unit.
6 See Appendix A, Note 6,
Secondary antibody incubation.