Hoefer PR625 Immunoblot - Manual (Page 6)

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p4
Aspirate and introduce antibody solutions
1
Aspirate excess liquid from the channels through the
numbered holes.
Note: To avoid drying of the membrane, channels
should be loaded within five (5) minutes of aspirating.
2
Pipette antibody solution through the numbered holes.
Press the pipette tip firmly into each hole and inject
the liquid rapidly in a single smooth action until the
channel is filled. Take care not to introduce bubbles.
3
3
Add buffer to all unused channels that cover the
membrane.
number of approximate
model channels channel volume 4
ImmunoBlot 25 250 µl
ImmunoBlot XL 45 140 µl
Incubate
1
Place the unit on a rocking platform with the channels
aligned in the direction of rocking.
Note: For best results, use a slow rocking speed (5 to
6 tilt cycles per minute). A gyratory shaking platform
is not effective for this purpose.
2
Incubate the unit on the rocking platform for 30 to
60 minutes at room temperature.
Recommendation: Use
a disposable pipette tip
connected to a water
vacuum aspirator for this
purpose.
Important! Take care not
to touch the pipette tip
anywhere on the unit except
in the desired sample hole.
3 See Appendix A, Note 3,
Eliminating bubbles.
4 See Appendix A, Note 4,
Optimal volumes.