Hoefer PR625 Immunoblot - Manual (Page 2)

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Introduction ..........................................................1
Unpacking and inventory ....................................2
Instructions ...........................................................3
Select and block membrane ...............................3
Load membrane ................................................3
Aspirate and introduce antibody solutions ............4
Incubate ...........................................................4
Remove primary antibody solutions
and wash the blot ..............................................5
Introduce secondary antibody and incubate .........6
Remove and develop the blot..............................7
Clean the ImmunoBlot .......................................7
Troubleshooting .....................................................8
Appendix A: Technical notes .................................10
Appendix B: Detection using ECL Western
blotting detection systems ....................................16
Ordering information ............................................18