Hoefer SE250 - Manual (Page 19)

3.5 Final assembly
1
Fill the lower buffer chamber with running buffer. The
SE250 holds about 150 mL. Check that the lower
electrode (running along the bottom of the the upper
buffer chamber core) is completely submerged.
2
Place the safety lid on the unit.
3
Plug the color-coded leads into the jacks of an
approved power supply such as the PS300B. The red
lead plugs into the red output jack, and the black
lead plugs into the black output jack.
4
Optional cooling: Begin circulating cold water or a
chilled 50/50 water/ethylene glycol solution.
•
p11
Important! Do not use antifreeze
or any alcohol-based mixture,
as these will irreparably damage
the core.
Note: If using precast gels,
check that the lower gel/buffer
contact surface is exposed.