Hoefer SE250 - Manual (Page 12)

3. Operating Instructions
3.1 Prepare the gel sandwich
Both precast and self-cast gels can be run in the
SE250 units. This unit accepts gels in 10 × 8 cm
plates, which can be cast in a Hoefer SE215,
SE245, or SE275 gel caster.
Each unit includes notched alumina plates and
rectangular glass plates. If casting your own
polyacrylamide gels, we recommend using a
notched alumina ceramic back plate because it
transfers heat 40 times more rapidly than glass.
For applications that are not heat sensitive, a
notched glass plate is available.
Before loading gels into the electrophoresis unit,
the separating gel should already be completely
polymerized. Clean away any gel adhering to
the notched alumina back plate. The stacking
gel (if applicable) can be cast in place on the
electrophoresis unit. Load liquid samples after
the gel sandwich is installed.
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p4
Notched plate
Note: All electrophoresis
accessories and kits are listed in
the the ordering section.
Note: Inspect glass plates
for chipped edges. Use only
unchipped plates to prevent
leaking.