Labconco Protector Controlled Atmosphere Glove Box - Manual (Page 27)

Chapter 3: Getting Started
Product Service 1-800-522-7658 23
Protector Fiberglass CA Glove Box
Oxygen Permeation at -4" H2O
ISO 10648-2 Test Method
0
20
40
60
80
100
120
0 5 10 15 20 25 30 35 40
Oxygen ppm
Ti
me
M
inute
s
Barometric Pressure
29.61 inches of Hg
Main Chamber
Pressure: -4 Inches
H
2
O
Transfer Chamber
Pressure: 0 inches of
Hg
Leak Rate Box
8.7x10¯ 5
Class 1 < 0.0005
Permeation
0.30ppm/min
Figure 3-10
The validation requirements and required testing instruments for all
applications shall be determined by the end user and Safety Officer. Other
validation test methods such as pressure leak decay tests may be conducted per
ISO 10648-2 standards. The pressure leak decay test may be performed, but
the pressure inside the glove box must be adjusted accurately for temperature
and barometric pressure.
These pressure leak decay tests are conducted under negative pressure of
-250 Pa (-1" water gauge) for operational use or -1000 Pa (-4" water gauge)
for the acceptance test; the pressure leak decay tests are usually performed
under stable barometric pressure and temperature conditions to minimize the
effect of these variables.
A 0.1°C fall in temperature results in an equivalent pressure change of -34
Pa or -0.14 inches of water gauge. A 0.01 inches of mercury rise in
barometric pressure results in an equivalent pressure change of -34 Pa or
-0.14 inches of water gauge.