Establishing an anaerobic environment, Establishing an anaerobic atmosphere, E 34 – Shellab BACTRON600 User Manual
Page 34: Operation (continued), Stablishing an, Naerobic environment
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P a g e
OPERATION (CONTINUED)
E
STABLISHING AN
A
NAEROBIC ENVIRONMENT
Carry out the following steps to purge the aerobic atmosphere from the workstation and establish
an anaerobic environment. If the BACTRON has previously been in use, remove the right arm port
sleeve prior to starting the procedure. This procedure requires approximately 1 to 4 hours to
complete.
1. Verify the following:
a. The interior and exterior pass box doors are closed and secured.
b. The manometer is filled to the fill line (upper ring) with water.
c. The foot pedal assembly center line is securely connected to the brass barb
adaptors on both arm port door assemblies.
d. The foot pedal assembly GAS and VAC lines are connected to the GAS OUT
and VAC OUT ports on the BACTRON.
e. Gas input lines are attached to the BACTRON
’
s GAS IN 1 and GAS IN 2 ports.
2. Note the reading on the AMG gas regulator.
a. The BACTRON300 (BACTRONII) will require approximately 200 psi of AMG
for a setup purge.
b. The BACTRON600 (BACTRONIV) and BACTRON900 (BACTRONIV-900)
need around 400 psi of AMG for a setup purge.
3. Make sure the AMG cylinder regulator is set to 15 to 20 psi. Open the valve all the way to
start a flow of gas to the BACTRON
a. Nitrogen and other inert gasses should not be used to purge the workstation.
4. Turn on the workstation, if it is not already powered up.
5. Open the right arm port door.
6. Verify that all incubator doors are slightly open to avoid
leaving reservoirs of aerobic atmosphere.
7. Unwrap and install a fresh catalyst cartridge on the left wall
inside the workspace chamber. See Figure 22 to the right.
a. Catalyst cartridges come ready for use from
the factory. However, if you have stored the
cartridges for longer than six months, it is
strongly recommended that you reactivate prior
to first use. Please see
on page 45.
b. The catalyst cartridge will grow warm in the presence of
oxygen and AMG.
Procedure continued on next page
Figure 22: Catalyst Cartridge
Installed