Gas source, Installation (continued), Ource – Shellab BACTRON600 User Manual
Page 14

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P a g e
INSTALLATION (CONTINUED)
G
AS
S
OURCE
Warning
: Never exceed a 5% hydrogen concentration insi
de the unit’s chamber.
Avertissement:
La concentration d’
hydrogène ne doit pas dépasser
5%
dans la chambre anaérobie.
The BACTRON requires an anaerobic gas mixture with 5% hydrogen to drive the oxygen-capturing
catalytic process in the workspace chamber. To reduce anaerobic gas consumption during auto
cycle pass box purges, the BACTRON may also be connected to a second compressed gas
cylinder containing an inert gas such as, nitrogen (N
2
), for use in the first two stages of the three-
stage auto cycle. Manual cycles of the pass box will only draw from the anaerobic mixed gas
cylinder attached to the GAS 1 In port.
For a single cylinder of anaerobic mixed gas (AMG), Sheldon Manufacturing recommends
5% hydrogen (H
2
), 5% Carbon Dioxide (CO
2
), and 90% Nitrogen (N
2
).
If you are using a two gas anaerobic application, Sheldon recommends one cylinder of
AMG gas at the above ratios, and a second cylinder of 100% Nitrogen (N
2
).
Gas regulators for BACTRON applications will need to be set at 15 - 20 psi. Please see
on page 49 in the Operation Section for working with
units other than Pounds per Square Inch.
Anaerobic Mixed Gas is often sold by gas suppliers under the category of Anaerobic Incubation
Mixtures or Biological Atmospheres. Contact your site safety officer and review your institutional
safety protocols for handling, storing, and using compressed gasses. Follow all local ordinances
and national regulations regarding compressed gases in research, clinical, or production
environments.