ANKOM 200 Series - Manual (Page 36)

Operator’s Manual
pg. 36
Rev C 1/14/15
Calculations
% Crude Fiber = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Weight of Organic Matter (loss of
weight on ignition of bag and
fiber)
C
1
= Ash corrected blank bag factor
(running average of loss of weight
on ignition of blank bag/original
blank bag)
11. Open the Vessel Lid and insert the Bag Suspender with bags
into the Vessel and place the Bag Suspender Weight on top
of the empty 9
th tray to keep the Bag Suspender submerged.
12. When processing 24 sample bags, add 1900-2000 mL of
ambient temperature AD solution to the fiber analyzer
vessel. If processing less than 20 bags, add 100 mL/bag of
AD (0.255N H
2
SO
4
) solution (use minimum of 1500 mL to
ensure Bag Suspender is covered).
13. Turn Agitate and Heat ON and confirm agitation.
14. Set the timer for 40 minutes and close/seal the lid.
15. When the extraction is complete, turn Agitate and Heat OFF.
16. Open the drain valve (slowly at first) and exhaust the hot
solution before opening the Vessel Lid. NOTE: The solution
in the Vessel is under pressure. The exhaust valve needs to
be opened to release the pressure and solution prior to
opening the Vessel Lid.
17. After the solution has been exhausted, close the exhaust
valve and open the Vessel Lid. Add 1900-2000 mL of 50-
90°C rinse water. Turn Agitate on and rinse for 5 minutes. If
the Heat is ON, the Vessel Lid should be closed. If the Heat
is OFF, the Vessel Lid can be open. Repeat 5 minute hot
water rinse 1 more time for a total of 2 rinses.
18. When processing 24 sample bags, pour 1900 - 2000 ml of
ambient temperature base (0.313N NaOH) solution over the
Bag Suspender in the Vessel. If processing less than 20 bags,
add 100 ml/bag of the base solution (minimum of 1500 ml).
19. Turn Agitate and Heat ON and confirm agitation.
20. Set the timer for 40 minutes and close/seal the lid.
21. When the extraction is complete, turn Agitate and Heat OFF.
22. Open the drain valve (slowly at first) and exhaust the hot
solution before opening the Vessel Lid. NOTE: The solution
in the Vessel is under pressure. The exhaust valve needs to
be opened to release the pressure and solution prior to
opening the Vessel Lid.
23. After the solution has been exhausted, close the exhaust
valve and open the Vessel Lid. Add 1900 mL of 50-90°C
rinse water. Turn Agitate on and rinse for 5 minutes. If the
Heat is ON, the Vessel Lid should be closed. If the Heat is
OFF, the Vessel Lid can be open. Repeat 5 minute hot water
rinse 2 more time for a total of 3 rinses.
24. When the Crude Fiber rinsing processes are complete, open
the Vessel Lid and remove the samples. Gently press out
excess water from the bags. Place the bags in a 250ml
beaker and add enough acetone to cover the bags and soak
for 3-5 minutes.
CAUTION4: Acetone is extremely flammable. Avoid static
electricity. A fume hood should be used at all times when
using acetone.
25. Remove the filter bags from the acetone and place them on a
wire screen to air-dry. Completely dry in an oven at 102 ±
2°C. (In most ovens the filter bags will be completely dry
within 2-4 hours.) NOTE: Do not place bags in the oven
until the acetone in the bags has completely evaporated.
26. Remove the filter bags from the oven and immediately place
them directly into a collapsible desiccant pouch and flatten
to remove any air. Cool to ambient temperature and weigh
the filter bags. NOTE: Do not use a conventional desiccator
container for this step.
27. Ash the entire filter bag/sample in a pre-weighed crucible
for 2 hours at 600 ± 15°C, cool in a conventional desiccator
and weigh to calculate loss of weight of organic matter (W
3
).