Operator’s manual – ANKOM 200 Series User Manual
Page 15
Operator’s Manual
Rev C 1/14/15
pg. 15
ADF Analysis Procedure using the ANKOM
200
Fiber Analyzer
To perform the ADF analysis on prepared samples, follow the procedure detailed below.
The Vessel must be at room temperature before beginning an ADF analysis.
1. Verify that the Exhaust Hose is connected to the instrument and securely positioned in the drain.
2. Turn the instrument Power Switch to the ON position.
3. Lift the Lid Handle to open the Vessel Lid.
4. If the temperature on the Temperature Controller display is higher than room temperature, fill the Vessel
with cold tap water. The temperature on the Controller will decrease. When the value on the Controller
reaches its lowest number and starts to increase, open the Exhaust Valve and exhaust the water. Repeat
this process until the number on the Temperature Controller equilibrates to room temperature.
5. Place the Bag Suspender with the samples and
the Bag Suspender Weight into the Vessel.
6. Pour a maximum of 2 L of Acid Detergent
solution into the Vessel.
7. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons on the
front of the instrument so they are lit. Look in
the Vessel to confirm agitation.
8. Close the Vessel Lid.
9. On the Timer/Clock, set the Timer to 60
minutes and press the Start/Stop button.
10. When the Timer sounds, the digestion process is
complete. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons
to turn them off (the buttons will not be lit when
they are off). Open the Exhaust Valve (slowly at
first) and exhaust the hot solution BEFORE
opening the Vessel Lid.
Because the Vessel is under pressure, the Exhaust Valve must be opened to
release the pressure and the solution BEFORE opening the Vessel Lid.
11. After the solution has been exhausted, open the Vessel Lid slowly to allow air to push any remaining
liquid out the Exhaust Hose.
12. Close the Exhaust Valve.
13. Add 1900 ml - 2000 ml of 70°C - 90°C rinse water.
During the rinse process, if the
H
H
E
E
A
A
T
T
button is OFF, the Vessel Lid can be
open. If the
H
H
E
E
A
A
T
T
button is ON, the Vessel Lid must be closed.
14. Press the
A
A
G
G
I
I
T
T
A
A
T
T
E
E
button.
15. On the Timer/Clock, set the Timer to 5 minutes and press the Start/Stop button.
16. When the Timer sounds, the rinse is complete. Slowly open the Exhaust Valve to drain the hot water.
Agitate Button
Heat Button
Timer/Clock
Temperature
Controller
Power Switch
(out of view)
Exhaust Hose Connector
Exhaust Valve Handle
Lid Handle
Vessel Lid
Vessel