ANKOM 200 Series - Manual (Page 22)

Operator’s Manual
pg. 22
Rev C 1/14/15
5. Place an empty filter bag in the Bag Holder in an open position.
6. Tare the weight of the empty filter bag and the holder together.
7. Add 0.95 – 1.00g of sample to the filter bag. Keep all particles away from the
sealing area of the filter bag.
8. Record the weight of the sample (W
2
).
9. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 – 3 seconds after the red
sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of
the filter bag. If the seal is not strong, re-seal the bag.
10. To eliminate sample clumping, spread the sample out uniformly within the filter bag by shaking and
flicking the bag.
11. Repeat steps 5 – 10 for all filter bags that will be used in the Analyzer. (Up to 24 bags can be processed
during one procedure with one of the bags being a blank.)
For all samples you will need to do a pre-extraction of fat before doing a
Crude Fiber analysis in the ANKOM
200
. Follow the pre-extraction steps
below:
1. Place the filter bags with sample into a 250ml container.
2. Pour enough petroleum ether into the container to cover the bags.
3. Allow the bags to soak for 10 minutes.
4. Pour out and dispose of the petroleum ether.
5. Place the bags on a wire screen to air-dry.
12. Place the filter bags with sample and at least one empty bag (used as a Blank) into
the Bag Suspender trays as shown (maximum of three bags per tray).
13. Stack each tray on the Bag Suspender rod (eight trays in total) with each tray
rotated 120 degrees from the tray below.
You must use all eight trays even if they are empty.
14. Add the ninth tray to the top of the Bag Suspender rod. This tray contains no filter bags and acts as a
cover.
The samples are now ready for the Crude Fiber analysis procedure.