Operator’s manual – ANKOM 200 Series User Manual
Page 23
Operator’s Manual
Rev C 1/14/15
pg. 23
Crude Fiber Analysis Procedure using the ANKOM
200
Fiber Analyzer
To perform Crude Fiber analysis on prepared samples, follow the procedure detailed below.
The Vessel must be at room temperature before beginning a Crude Fiber
analysis.
1. Verify that the exhaust hose is connected to the instrument and securely positioned in the drain.
2. Turn the instrument Power Switch to the ON position.
3. Lift the Lid Handle to open the Vessel Lid.
4. If the temperature on the Temperature Controller display is higher than room temperature, fill the Vessel
with cold tap water. The temperature on the display will decrease. When the value on the display
reaches its lowest number and starts to increase, open the Exhaust Valve and exhaust the water. Repeat
this process until the number on the Temperature Controller equilibrates to room temperature.
5. Place the Bag Suspender with the samples and
the Bag Suspender Weight into the Vessel.
6. Pour a maximum of 2 L of ambient
temperature acid (0.255N H
2
SO
4
) solution
into the Vessel.
7. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons on the
front of the instrument so they are lit. Look in
the Vessel to confirm agitation.
8. Close the Vessel Lid.
9. On the Timer/Clock, set the Timer to 40
minutes and press the Start/Stop button.
10. When the Timer sounds, the digestion process
is complete. Press the
H
H
E
E
A
A
T
T
and
A
A
G
G
I
I
T
T
A
A
T
T
E
E
buttons to turn them off (the buttons will not
be lit when they are off). Open the Exhaust
Valve (slowly at first) and exhaust the hot
solution BEFORE opening the Vessel Lid.
Because the Vessel is under pressure, the Exhaust Valve must be opened to
release the pressure and the solution BEFORE opening the Vessel Lid.
11. After the solution has been exhausted, open the Vessel Lid slowly to allow air to push any remaining
liquid out the Exhaust Hose.
12. Close the Exhaust Valve.
13. Add 1900 ml - 2000 ml of 50°C - 90°C rinse water.
During the rinse process, if the
H
H
E
E
A
A
T
T
button is OFF, the Vessel Lid can be
open. If the
H
H
E
E
A
A
T
T
button is ON, the Vessel Lid must be closed.
14. Press the
A
A
G
G
I
I
T
T
A
A
T
T
E
E
button.
15. On the Timer/Clock, set the Timer to 5 minutes and press the Start/Stop button.
Agitate Button
Heat Button
Timer/Clock
Temperature
Controller
Power Switch
(out of view)
Exhaust Hose Connector
Exhaust Valve Handle
Lid Handle
Vessel Lid
Vessel