Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 19)

Technical Support: 1-800-424-6723 • www.bio-rad.com 15
Section 5
Troubleshooting Guide
Fault Possible cause Action
Foresight ™
RoboColumn
units are
clogged
• The sample is too
viscous
• There is too much
cell debris in the
sample
• The column has
been regenerated
and used for too
many cycles
• Increase dilution of
the cell isolate before
lysis, or dilute after
the lysation
• Centrifuge and/or
filter the sample if
unclarified sample
has been used
• Reduce the number
of cycles
Foresight
RoboColumn
units are leaking • Columns are
clogged
• The O-ring seal is
damaged
• The insertion depth
of the robotic needle
is incorrect
• See above
• Check the taught
X and Y coordinates
for RoboColumn
labware for precision
• Check the insertion
depth (16 mm
from the top of
the column) of the
robotic needle
Foresight
RoboColumn
units are lifted
up from the
96-well array
plate during the
pull-out of the
robotic needles Columns are not securely
seated into the 96-well
array plate Make sure that all
columns are securely
seated into the
96-well array plate by
firmly pressing down
before starting the
experiment
Low protein
recovery in the
elution fraction(s) The residence time for
sample loading is too low Decrease the flow rate or
increase residence time
for sample loading