Bio-Rad Foresight™ Chromatography Columns, Prepacked User Manual
Page 17
Technical Support: 1-800-424-6723 • www.bio-rad.com 13
After the experiment setup the Te-Chrom Wizard will generate a
chromatographic method in Freedom EVOware
software automatically.
f. Reporting
•
Set output folder
•
Select output format
6. Run chromatographic method
Note: Make sure all required
solvents and consumables
such as collection plates are in
their designated positions on
the robotic worktable before
running the chromatographic
method.
7. Data evaluation
a. Determine the precise
volume of the collected
fractions by using an
appropriate plate reader
b. Determine the protein
concentration by
measuring UV
Note: After path length
detection with the RoMa,
transfer the collection
plate to any location on
the robotic worktable, or
to a secondary device
for further analysis such
as HPLC, MS, ELISA, or
CGE/SDS-Page.