Bio-Rad Foresight™ Chromatography Columns, Prepacked - Manual (Page 10)

6 Technical Support: 1-800-424-6723 • www.bio-rad.com
Table 3. Recommended equipment for automated handling of
Foresight RoboColumn units, continued.
Equipment Details Tips and tricks
Reagent and
sample
reservoirs A broad spectrum
of troughs, tubes,
and plates can be
positioned onto the
robotic worktable
by using the
appropriate Tecan
carrier Use an 8-, 48- or 96-
deep well reservoir for
buffer
Collection plate Use any type of
multi-well plate
which matches the
ANSI/SDS
standard (UV or
non-UV readable) • Use a half area
96-well microplate to
collect fractions in a
volume range from
25–175 µl
• Use a standard 96-
well microplate to
collect fractions in a
volume range from
75–340 µl
• Use 96-deep well
reservoirs to collect
high volume fractions
up to 2.0 ml
3.2 Sample Preparation
Loading a clarified sample onto Foresight
™ RoboColumn units is
recommended because unclarified sample may cause clogging of
the top and bottom filter frits. This may impact the performance of
the column.
3.3 Experiment Setup
Foresight RoboColumn units are suitable for applications such as
parallel screening and optimization of chromatographic conditions
in downstream process development. The flexible design enables
arrangement of up to 12 rows (8 columns per row) of Foresight
RoboColumn units on a 96-well array plate (available upon request)
according to application’s requirements.