Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 15)

7. Plot all 8 spectra and select the IgG peak using the peak selection
tool.
Note: Spots with high concentrations (spots A, C, E, and G) alternate
with those with low concentrations (spots B, D, F, and H). Due to the
different concentrations on different spots, it is critical to export the peak
data with the spots in alphabetical order from A to H.
8. Export the data according to the instructions in Appendix A and
selecting parameters for export in the following order:
• Array bar code
• Spot name
• Substance mass
• Intensity
• Resolution
• S/N
9. Paste the data into the ProteinChip system check form, data sheet
“Test 1 Detector Sensitivity”.
10. The spreadsheet indicates if the test passed or failed.
11. If test 1 fails, run the detector calibration procedure (maintenance
procedure 2), and repeat the test. If the test fails a second time,
contact technical support.
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