Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 11)

Note: The detector calibration procedure is intended to standardize
instrument performance over time. First-time use may alter system
response and is not recommended within a series of experiments. It is,
however, possible to manually set the voltage back to its original state
through the instrument’s web page.
1. Insert the ProteinChip detector calibration array into the instrument.
Note: For the Enterprise Edition instrument, place the array into slot 1
of a cassette and fill the rest of the slots with blank or used ProteinChip
arrays.
2. Open the instrument’s interactive web page (http://pcs4000-
####/index.jsp, where #### is the instrument serial number). If the
instrument is under local control, the web page will only be on the
local computer. If it is installed on an intranet, it will be available on
all intranet computers.
3. Select Calibrations > Automatic Detector Gain. The Detector
Gain page opens. Check that Automatic and NOT manual is
selected.
4. If using the Enterprise Edition instrument, select Array number 1
(slot 1 in cassette).
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