Bio-Rad ProteinChip Matrices - Manual (Page 6)

© 2006 Bio-Rad Laboratories, Inc.
Applying EAM to Arrays
The method of ProteinChip EAM application to the
array surface requires practice as it can significantly
influence the quality of the data. A minute volume
(0.3–1.0 µl) of ProteinChip EAM in organic solvent must
be applied to each sample prior to analysis. It is
recommended that ProteinChip EAM be applied to the
spots after they have dried completely for the greatest
spot-to-spot consistency.
If variable signal is seen across the spot when reading
the arrays, it may be useful to observe the
ProteinChip EAM in good light, or under a dissecting
microscope. The EAM should be distributed fairly
uniformly across the spot surface.
Note:
1. When pipetting ProteinChip EAM onto samples, be careful to avoid
the undissolved EAM at the bottom of the tube.
2. Always use new, packaged pipet tips; never use tips that have
been autoclaved or reused.
Storage
ProteinChip EAM are light-sensitive and should be
stored away from the light.