Bio-Rad ProteinChip Matrices User Manual

Page 3

ProteinChip SPA EAM give better resolution and fewer
multiply charged ions than ProteinChip EAM-1 EAM.
For protein profiling using only one kind of EAM,
ProteinChip SPA EAM are the molecules of choice.

ProteinChip EAM-1 EAM, 5 mg/vial

ProteinChip EAM-1 EAM are proprietary molecules
(MW 231.21) and work well for proteins in the 10–50 kD
mass range. ProteinChip EAM-1 EAM prove useful in
allowing desorption and ionization of proteins that are
difficult to detect, such as glycosylated proteins. It
should be noted that ProteinChip EAM-1 EAM tend to
generate multiply charged species, and peaks tend to
be broader due to more adduct formation.

Preparing ProteinChip EAM in Solution

ProteinChip EAM in solution can be prepared in a
number of different ways that may optimize the
detection of the proteins in your sample. Table 1 to the
right lists the steps for preparing EAM in solution using
commonly used solvent systems. For a comprehensive
list of solvent solution options, please see the
ProteinChip SELDI System Applications Guide,
Volume 1.