Bio-Rad ProteinChip Matrices - Manual (Page 4)

Table 1. Preparation of ProteinChip EAM in solution using
commonly used solvent systems.
EAM Solvent System Preparation
ProteinChip CHCA 50% acetonitrile
49.75% HPLC
grade H
2
O
0.25% trifluoroacetic
acid (TFA)
ProteinChip SPA 50% acetonitrile
49.50% HPLC
grade H
2
O
0.50% TFA
ProteinChip EAM-1 50% acetonitrile
49.50% HPLC
grade H
2
O
0.50% TFA
© 2006 Bio-Rad Laboratories, Inc.
Preparation
1. Add 200 µl solvent to vial
(there should be undissolved
CHCA in the bottom of the
tube)
2. Mix
3. Centrifuge
4. Draw off supernatant and
dilute with equal part solvent
1. Add 400 µl solvent to vial
2. Mix
3. Centrifuge
1. Add 200 µl solvent to vial
(there should be undissolved
EAM-1 in the bottom of the
tube)
2. Mix
3. Centrifuge
4. Draw off supernatant and
dilute with 2x volume of
solvent