Bio-Rad Fluorescent Assay Kits - Manual (Page 6)

rinse their surfaces and prevent buffer carry-over into the sample
vial (see Table 2).
Sample Injection—The best resolution and sensitivity are normally
obtained using electrophoretic injection at constant voltage and a
current limit of 100 µ A. However, in cases where the sample
contains excessive amounts of salt, pressure injection may
provide better results. Because of the viscosity of the run buffer,
a pressure injection value of 100 psi*sec or more should be used;
this injects a volume of approximately 15 nl.
3.3 Analysis of Multiple Samples
For analysis of multiple samples, a fresh set of run buffer vials
should be used every 30 injections.
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Table 1. BioFocus Configuration for dsDNA Analysis
ID: DNA Description: dsDNA Analysis
INLET CAROUSEL POSITIONS Amount
Pos Type ID/Description Contents Vial Size in Vial
1 R DNA_RUN Run buffer 500 µl 500 µl
2 R DNA_PREP Run buffer 500 µl 500 µl
3 R WATER_DIP Water 500 µl 500 µl
4 R WATER_DIP Water 500 µl 500 µl
9 R WATER/Shutdown Water 500 µl 500 µl
10 R NITROGEN/Shutdown Empty 500 µl
11 S 100 bp ladder Sample 500 µl 20–50 µl
OUTLET CAROUSEL POSITIONS Amount
Pos Type ID/Description Contents Vial Size in Vial
1 R DNA_RUN Run buffer 500 µl 500 µl
2 R WATER/Shutdown Water 500 µl 500 µl
32 W WASTE Water (100 µl) 500 µl 100 µl
CARTRIDGE DATA
Catalog Number: UAC Serial Number: DNA
Length: 24 cm Diameter: 75 mm Coated
Use Count: 0 Active
3.2 dsDNA Analysis Method
Preparation Cycles—The 45 second, high pressure preparation cycle
(Prep cycle 1) fills the capillary with fresh run buffer at the begin-
ning of each analysis. The 0 second cycles (Prep cycle 2 and 3) dip
the capillary and electrode into vials containing deionized water to
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