Bio-Rad Fluorescent Assay Kits - Manual (Page 3)

Section 1
Introduction
The CE dsDNA 1000 Fluorescent Detection and Analysis Kit
is optimized for use with the BioFocus ® capillary electrophoresis
laser induced fluorescence LIF 2 detection system and resolves
double-stranded (ds) DNA fragments ranging in size from 100 to
1,000 base pairs. The kit is useful for a variety of applications
including determination of PCR
* yields, optimizing PCR conditions,
and characterizing restriction enzyme digests. High performance
capillary electrophoresis is an ideal approach for nucleic acid
separations, providing rapid analysis, quantitative information,
minimal consumption of sample, and direct detection of resolved
fragments.
High resolution DNA separations are achieved by dynamic
sieving ** capillary electrophoresis, a technique that incorporates a
hydrophilic polymer in the electrophoresis buffer. The polymer-con-
taining buffer is replenished between each analysis, providing
reproducibility of migration time and peak area. The analysis
employs BioCap
™ capillaries which are coated internally with a
novel polymer (polyAAEE), insuring high resolution separations
over many runs.
SYBR ® Green I, the fluorescent dye included in the kit, is an
intercalating monomeric dye with the excitation maximum close
to the 488 nm line of the argon ion laser, extremely low intrinsic
fluorescence, and very high affinity for dsDNA.
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