Bio-Rad Fluorescent Assay Kits - Manual (Page 2)

Table of Contents
Section 1 Introduction ..............................................................1
Section 2 CE dsDNA 1000 Fluorescent Detection and
Kit Components......................................................2
2.1 Capillary Preparation and Use ..............................................2
2.2 Using the CE dsDNA 100 Base Pair Ladder ..................3
2.3 Sample Preparation ..........................................................4
2.4 Buffer Preparation for Resolving Fragments
of 100–1,000 Base Pairs ........................................................4
Section 3 Analysis Conditions on the
BioFocus ® System ......................................................5
3.1 Configuration......................................................................5
3.2
dsDNA Analysis Method ..................................................6
3.3 Analysis of Multiple Samples ............................................7
Section 4 System Shutdown Method......................................8
Section 5 Optimization of Nucleic Acid Separations ........10
5.1 Shortening Analysis Time................................................10
5.2 Increasing Resolution ......................................................10
5.3 Regenerating a Plugged Capillary ..................................11
Section 6 Product Information ............................................12
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