Bio-Rad Rotofor® and Mini Rotofor Cells - Manual (Page 43)

Bulletin # Title
Detergents or Denarurants used
1518 Purification of Bacterial Eukaryotic Fusion Proteins Using the
Rotofor Cell
1517 Rotofor Fractionation of Intestinal Brush Border Membrane
Proteins
1514 Isolation of a Membrane Bound Immunoregulatory Molecule from
Metastatic Lymphoma Cells
1515 Preparation of Spinach Cold Acclimation Proteins for Gas Phase
Sequencing, Oligonucleotide Derivation and Monoclonal Antibody
Production
1475 Isolation of Monoclonal Antibodies to Phencyclidine from Ascite
Fluid
Section 13
Application for Preparative Two Dimensional
Electrophoresis System
Preparative electrofocusing in the Rotofor Cell is typically used for the initial
fractionation of proteins from crude mixtures. Following primary purification in the
Rotofor Cell, a final purification step may be needed to isolate a specific component.
Bio-Rad's Model 491 Prep Cell is a continuous elution polyacrylamide gel
electrophoresis device that is designed to be used as a secondary, and final
purification step following the Rotofor Cell. The following example illustrates the
usefulness of Bio-Rads unique "preparative two-dimensional electrophoresis system".
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