Bio-Rad Rotofor® and Mini Rotofor Cells User Manual

Page 13

3.3 Assemble the Focusing Chamber

1. Slide the assembled anode electrode assembly over the ceramic cooling finger

so that the two protruding screw heads fit into the holes in the black plastic
base of the cooling finger support assembly.

Fig. 3.3. Anode electrode assembled on the cooling finger.

2. Slide the membrane core onto the ceramic cooling finger, making sure the core

abuts the acrylic ridge on the anode chamber.

3. Slide the focusing chamber over the membrane core, inserting the metal pin

into the small hole in the anode chamber. Position the focusing chamber so
that each membrane screen lies between two adjacent ports. These ports must
not be blocked by the membrane screens at either side, load or harvest. If the
ports are blocked, remove the focusing chamber, and slide it once more over
the membrane core. Tighten the black, nylon retaining screws. Check again to
make sure the membrane screens do not block the ports of the chamber.

Fig. 3.4. Slide the focusing chamber over the membrane core.

4. Slide the assembled cathode compartment over the cooling finger, aligning the

metal pin and hole in the cathode chamber, and tighten the nylon retaining
screws.