Bio-Rad Rotofor® and Mini Rotofor Cells User Manual
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Section 11
Rotofor References
Methods
Whether alone or in combination with other techniques- polyacrylamide gel
electrophoresis (PAGE), electroelution, or chromatography, for example- the
Rotofor system integrates into any purification scheme. The following articles
illustrate the use of the Rotofor in a variety of different protein purification and
enrichment methods.
Westman-Brinkmalm A, Davidsson P (2002)
Comparison of preparative and analytical two-dimensional electrophoresis for isolation and
matrix-assisted laser desorption/ionization-time-of-flight mass spectrometric analysis of
transthyretin in cerebrospinal fluid.
Anal. Biochem. 301: 161-7.
Madoz-Gurpide J, Wang H, Misek DE, Brichory F, Hanash SM (2001)
Protein based microarrays: a tool for probing the proteome of cancer cells and tissues.
Proteomics. 1(10):1279-87.
Hesse C, Nilsson CL, Blennow K, Davidsson P (2001)
Identification of the apolipoprotein E4 isoform in cerebrospinal fluid with preparative
two-dimensional electrophoresis and matrix assisted laser desorption/ionization-time of
flight-mass spectrometry.
Electrophoresis. 22(9): 1834-7.
Davidsson P, Paulson L, Hesse C, Blennow K, Nilsson CL (2001)
Proteome studies of human cerebrospinal fluid and brain tissue using a preparative
two-dimensional electrophoresis approach prior to mass spectrometry.
Proteomics. 1(3): 444-52.
Gustafsson E, Thoren K, Larsson T, Davidsson P, Karlsson KA, Nilsson CL (2001)
Identification of proteins from Escherichia coli using two-dimensional semi-preparative
electrophoresis and mass spectrometry.
Rapid Commun Mass Spectrom. 15(6): 428-32.
* Nilsson CL, Larsson T, Gustafsson E, Karlsson KA, Davidsson P (2000)
Identification of protein vaccine candidates from Helicobacter pylori using a
preparative two-dimensional electrophoretic procedure and mass spectrometry.
Anal Chem. 72(9): 2148-53.
* Wall DB, Kachman MT, Gong S, Hinderer R, Parus S, Misek DE, Hanash SM, Lubman
DM (2000)
Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method
for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry.
Anal Chem. 72(6): 1099-111.
Nilsson CL, Puchades M, Westman A, Blennow K, Davidsson P (1999)
Identification of proteins in a human pleural exudate using two-dimensional preparative liquid-
phase electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry.
Electrophoresis. 20(4-5): 860-5.
* A reprint of this article is available in the Rotofor technical folder.
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