Bio-Rad ReadyPrep Protein Extraction Kit (Soluble/Insoluble) User Manual
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Section 3
Storage Conditions
Store the unopened kit at room temperature. After opening,
unused reconstituted ReadyPrep 2-D Rehydration/Sample
Buffer 1 should be aliquoted in 1 to 2 ml volumes and
stored frozen at –80°C. After opening, transfer the
ReadyPrep TBP reducing agent to the empty glass vial
provided and store the vial at –20°C to prevent evaporation
of the TBP. Aliquot and store unused Lysis Buffer at –20°C.
Section 4
Reagent Preparation
ReadyPrep 2-D Rehydration/Sample Buffer 1
:: Add
5.6 ml of ReadyPrep proteomic grade water or similar
quality water to one bottle. Swirl the vial gently until the
contents are completely dissolved. The solution can be
warmed slightly in the palm of the hand or in a water bath
to speed the dissolution process. DO NOT heat the
solution above 25 to 30°C to avoid the formation of
cyanates. Cyanates can react with and modify the proteins
in the sample.
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