Bio-Rad ReadyPrep Protein Extraction Kit (Soluble/Insoluble) User Manual
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Section 2
Kit Specifications
Each ReadyPrep protein extraction kit (Soluble/Insoluble)
provides sufficient reagents to perform up to 20 extractions
each starting with 100–200 mg of cells or tissue. The
procedure can easily be scaled up to accommodate larger
amounts of cells or tissue. The entire procedure can be
completed in less than 1 hr.
Items Supplied With Kit
• 2-D Rehydration/Sample Buffer 1. 2 vials. Lyophilized.
After reconstituting, each vial contains 10 ml of 7 M urea,
2 M thiourea, 1 % (w/v) ASB-14 detergent, 40 mM Tris
base, and 0.001% Bromophenol Blue.
• Lysis Buffer. 5 pouches. Each pouch reconstitutes to
make 50 ml of Lysis Buffer.
• TBP Reducing Agent. One ampoule containing 0.6 ml of
200 mM tributylphosphine (TBP) in 1-methyl-
2-pyrrolidinone sealed under nitrogen gas.
• Empty Vial. 1 storage vial for TBP reducing agent.
• Instruction Manual,1.
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