Bio-Rad ReadyPrep Protein Extraction Kit (Soluble/Insoluble) User Manual
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The ReadyPrep 2-D rehydration/sample buffer 1 is a key
component of this kit, being used for both protein
extraction as well as for IPG strip rehydration. Rehydration/
sample buffer 1 is a strongly chaotropic buffer containing
the zwitterionic detergent ASB-14, making this solution one
of the most powerful solubilizing reagents available for 2-D
electrophoresis. After making an appropriate dilution into a
rehydration/sample solution, the two protein fractions gen-
erated by this kit are suitable for isoelectric focusing on
Immobilized pH gradient strips (IPGs) and subsequent 2-D
gel separation.
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