Bio-Rad ReadyPrep Protein Extraction Kit (Soluble/Insoluble) - Manual (Page 13)

is available and sold separately for this purpose (see
Section 7).
Note: For some applications (for example using basic pH
range IPG strips), the protein sample may require reduction
and alkylation treatment before IEF in order to achieve opti-
mal 2-D separation. The ReadyPrep Reduction-Alkylation Kit
can be purchased separately for this purpose (catalog
#163-2090).
14. The samples are now ready to be loaded onto IPG
strips. Unused Soluble and Insoluble protein extracts
should be stored in aliquots at –80°C.
Note when working with plant leaf samples: for best 2-D
separation results treat the sample using the ReadyPrep
2-D Cleanup Kit (Bio-Rad catalog #163-2130) prior to
performing IEF.
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