Bio-Rad PureZOL™ RNA Isolation Reagent - Manual (Page 16)

Problem Possible Cause Recommended Solution
Low yield Insufficient amount of Scale up the amount of
(continued) PureZOL used for PureZOL used according to
sample lysis and sample size. Sample volume
homogenization should not exceed 10% of
the PureZOL reagent used
for homogenization
Lysate was not Make sure to incubate the
incubated at room lysate after the disruption
temperature for step for 5 minutes at room
5 minutes after the temperature to allow
disruption step (see complete dissociation of
step 4 in protocol) nucleoprotein complexes
RNA is RNase contamination Treat all user-made solutions
degraded of solutions supplied with DEPC before use (see
by the user section 3, Maintaining an
RNase-Free Environment)
RNase contamination See section 3, Maintaining
of plasticware and an RNase-Free Environment
work station
Frozen tissue samples Add PureZOL directly to
were allowed to thaw frozen samples before they
or sit at room thaw. Do not let starting
temperature samples sit at room
temperature
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