Bio-Rad AG® MP-50 Cation Exchange Resins - Manual (Page 8)

5.2 Column Method
The column method involves pouring a column with
the resin and passing the sample through to achieve the
separation. Particle size will determine the flow rate,
which will affect the separation. The resin should be in
the correct ionic form and equilibrated prior to adding
the sample.
1. Calculate the amount of resin required based on the
expected resin capacity and sample concentration. If
the sample ionic concentration is unknown, begin
with 5 grams of resin for 100 ml of sample, and then
optimize the volumes after obtaining the results.
2. Insure that the resin is in the ionic form which will
allow the sample ions to be exchanged onto the resin.
If conversion of the resin into another ionic form is
necessary, use the guidelines described above for
resin conversion (see Table 5).
3. Prepare the initial buffer, so that the pH and ionic
concentration will allow the sample ions to be
exchanged onto the column. For unknown solutions,
use deionized water.
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Section 5
Instructions for Use
AG 50 and AG MP-50 resin may be used in either a
batch method or a column method. The batch method
consists of adding the resin directly to the sample and
stirring. The column method requires preparing a column
filled with resin, and passing the sample through.
5.1 Batch Method
The batch method is performed by adding the resin
directly into the sample and stirring. The resin should be
in the correct ionic form prior to beginning.
1. Weigh out about 5 grams of resin for every 100 ml of
sample. For larger scale applications or when an
exact amount of resin is needed, calculate the resin
volume based on the resin capacity.
2. Add resin to the sample and stir or shake gently for 1
hour.
3. Filter or decant the sample from the resin.
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LIT203B 6/17/98 12:00 PM Page 10