Bio-Rad AG® MP-50 Cation Exchange Resins User Manual
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Section 8
Storage
The resins are stable for at least 2 years when stored
in the original, unopened container at room temperature
and protected from ultraviolet light.
Section 9
Stability
The resins are stable in acid, base, and organic
solvents, and may be autoclaved. To prevent bacterial
growth during prolonged storage of a poured column,
use a preservative such as 0.05% sodium azide or
thimerosol or 20% organic solvent such as methanol or
ethanol.
Table 7. Cation Exchangers for Sample
Preparation
Application
Resin
Reference
Cation removal from
AG 50W-X8 Ochiai, M.,
J. Chromatog., 194,
monosaccharides
resin
224 (1980).
Removal of cations
AG 50W-X8 Hoffer, E. M., Kothny, E. L. and
from sulfate
resin
Appel, B. R., Atmospheric
Environment, 13, 303 (1979).
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6.5 Notes
The experimental error is only that inherent in the
titration procedure. The error due to the ion exchange
itself is less than that of the titration. To avoid the error
due to interfering carbonate ions, neutralize alkaline tap
water with 0.1 M HCl, one drop at at time, to the methyl
orange end point.
The column may be used several times before
regeneration is necessary. To regenerate the resin, wash
it by passing approximately 50 ml of 3 M HCl through
the column, followed by 75 ml of distilled water.
Section 7
Applications
Strong cation exchange resins are used for sample
preparation, metal separations, weak acid separations,
peptide separations, amino acid separations, and nucleotide
separations. Tables 7-10 summarize the applications.
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