Bio-Rad Media Sampler Pack - Manual (Page 6)

Chemical Stability
Macro-Prep ion exchange media are stable in most aqueous buffer solutions
commonly used in purification of biomolecules. These ion exchangers also with-
stand treatment in solutions of acid, detergents, chaotropic agents, and pH < 10,
while retaining full functional performance. We do not recommend routine cleaning
or operation of Macro-Prep High Q and DEAE media above pH 10. Macro-Prep
ion exchange media should not be sanitized or stored in NaOH.
Table 2. Chemical Stability of Macro-Prep Ion Exchange Media:
Total Organic Carbon (ppm) Leachables.
Exposure High Q DEAE High S CM
0.1 M HCl 0 0 4 0
0.2 M H3PO4 0 0 13 1
0.01 M NaOH 28 N/A N/A N/A
0.1 M NaOH 200 26 31 4
1.0 M NaOH 337 121 49 6
Each of the media (10 ml) was exposed to the specific reagent (40 ml) and mixed for 24 hr at 23°C.
Extracts were analyzed on a Shimadzu total organic carbon analyzer, Model TOC 5000A.
Thermal Stability
Macro-Prep media can be autoclaved at 121°C for up to 30 min in deionized water as a
slurry or a moist cake. Do not autoclave Macro-Prep High Q and DEAE media in OH–
form.
Section 5
Preparation for Use
Wash the ethanol storage solution from the medium with deionized water.
Small volumes of Macro-Prep media are easily washed in a Büchner funnel with
4–5 column volumes of water or a low ionic strength (<20 mM) buffer. For large
volumes, it may be more convenient to pour the desired amount of medium into a
suitable container, allow the medium to settle, and decant the ethanol solution.
Add one column volume of deionized water, resuspend the medium, allow it to
settle, and decant the supernatant. Repeat this procedure with buffer (approxi-
mately 4–5 times, or until the ethanol is removed), and then pack the column (see
Column Packing). The medium may be prepared by decanting the excess ethanol
solution and resuspending the medium in the application buffer prior to column
packing (Column Packing, step 1).
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