Bio-Rad Media Sampler Pack - Manual (Page 4)

Section 3
Media Screening
Macro-Prep ion exchange media are supplied prepacked in 1 ml and 5 ml cartridges for
ease of screening. In addition, these media are part of the Media Sampler Pack, which
includes Macro-Prep HIC, UNOsphere
™ and CHT ™ ceramic hydroxyapatite media. (See
Ordering Information in Section 11 at the end of this instruction manual for a list of
available items.) For the most up-to-date package options, visit our web site at
www.bio-rad.com.
It is important with Macro-Prep ion exchange media to evaluate both conductivity and pH
conditions. Since ion exchange media are often used after an affinity step, it may be
necessary to adjust the salt concentration and/or the pH of the sample load to obtain
optimal purification and recovery with Macro-Prep ion exchange media.
Section 4
Technical Specifications and Characteristics
Macro-Prep ion exchange media are shipped as a 50% slurry in 20% ethanol. Table 1
lists the properties of the Macro-Prep ion exchange media. The rigidity of these media
allows high flow rates without bed compression (Figure 1). Testing in a small laboratory-
scale column (10 mm ID X 100 mm length, 7.9 ml) showed no bed compression at a
pressure of 7 bar and a flow rate of 40 ml/min (3,000 cm/hr). This property provides for
high flow rates used during loading, column washing, equilibration, and cleaning.
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