Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges User Manual
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•
Carefully transfer the slurry to the column (using the pre calculated volume).
Slurry can be transferred using a diaphragm pump or other gentle transfer
method
•
Allow the slurry to settle until a 2–5 cm layer of clear supernatant packing
buffer is observed above the bed
•
Replace the top flow adapter (piston assembly) and lower into supernatant
layer
•
Engage the seal. Lower the top piston in order to remove any trapped air
and purge the top piston assembly of air
•
Once all of the air is removed, begin flow packing at approximately 300 cm/hr.
This value may change depending on your process requirements
o
Columns equipped with speed-controlled dynamic axial compression
capabilities (such as Bio-Rad
®
EasyPack™ columns) can be packed
using a combination of flow packing and axial compression, or only
using axial compression
o
Columns without axial compression capabilities may be flow-packed
•
The optimal compression percentage for UNOsphere SUPrA™ media is
12%
4.2 Packing in Closed Column Systems
Using Bio-Rad
®
InPlace™ Columns
Bio-Rad InPlace columns are typically packed using a Bio-Rad
®
media transfer
device and a slurry tank. The slurry tank should be filled with slurry at 30–50%
concentration, and should be equipped with a low-shear impeller for gentle mixing.
•
The column should be cleaned and levelled with the piston lifted to the
uppermost position
•
Ensure that there is no air trapped in the bottom process valve or frit by
reverse flowing with packing buffer for a short period or by introducing
packing buffer into the column and allowing it to drain
•
Verify that about 2 cm of packing buffer remains at the bottom of the
column. Close the bottom process valve
•
The seal may be deflated prior to slurry transfer
•
Using the media transfer device, open the slurry valves. Open the bottom
valve on the slurry tank to allow the transfer of slurry into the InPlace
column. Turn on the diaphragm pump to increase the speed of transfer
•
After completing the slurry transfer, rinse the slurry tank and transfer lines
with reserved buffer. Close the slurry valves
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