Bio-Rad Bio-Scale™ Mini UNOsphere SUPrA™ Affinity Cartridges - Manual (Page 8)

4
3.1 Determining Slurry Percentage or Media Volume
The recommended slurry percentage for column packing is 30–50%. There are
several methods that can be used to determine the slurry percentage of a
solution.
Using disposable open columns (e.g. Bio-Rad Econo-Pac ® columns):
Method 1:
Fill the column with slurry and use a marker to indicate the filled height. Open
the bottom of the column and drain the buffer. Mark the bed height of the
remaining media. The height of the remaining media divided by the height of the
total slurry volume will yield the approximate slurry percentage.
Method 2:
Pour slurry into a graduated cylinder and allow to settle overnight (>12 hrs). The
height of the settled bed divided by the height of the total volume will yield the
approximate slurry percentage.
Method 3:
If the media are packed into a column, flow at 300 cm/hr with the top flow
adapter at least 10 cm above the packed bed. Stop the flow and open the top
of the column. Allow the bed to rebound for at least 15 min to determine the
bed volume prior to compression.
Section 4
Column Packing
General column packing procedures are outlined below for the two main
process scale column types (open and closed column systems). Please consult
the user guide of the specific column you are using for complete instructions.
See Figure 1 for the flow properties of UNOsphere SUPrA media.
Caution:
Some column systems require recirculation of slurry for extended periods
through the packing pump. Avoid this operation with UNOsphere SUPrA™
media, as vigorous recirculation can damage the media and create an excessive
amount of fine particles. Consult Bio-Rad Technical Services for alternate
methods.
4.1 Packing in Open Column Systems
• Completely remove top piston assembly. Make sure the column is level
• Ensure that there is no air trapped in the bottom process valve or frit by
performing a brief up-flow with packing buffer, or by pouring packing
buffer into the column and allowing it to drain
• Leave about 2 cm of packing buffer at the bottom of the column. Close
the bottom process valve
10014430B:4110109C.qxd 7/1/2009 11:43 AM Page 4