Bio-Rad Prepacked Gravity Flow Columns for Ion Exchange - Manual (Page 9)

can be removed easily by rinsing with 2-3
bed volumes of mobile phase.
3. Effluent is red: After prolonged storage,
sulfonic acid will sometimes leach from
AG 50 strong cation exchange resin, yield-
ing a red effluent. Wash the resin with
mobile phase or water until the color is no
longer visible.
4. Cloudy effluent: The initial rinse may con-
tain a small quantity of resin. Discard the
wash and continue as normal.
5. Flow rate too fast: Use a stopcock to adjust
the flow rate.
6. Column runs dry: If the resin is allowed to
become dry, air bubbles can form in the
column bed. This will affect the flow and
the separation efficiency. To correct this,
add extra mobile phase, stop-up both ends
of the column, shake the column, and
allow the resin to settle. Proceed as normal.
If you have any questions about using pre-
filled Poly-Prep columns, call our toll-free
technical services number, 1-800-424-6723 (in
the U.S.), or contact your local Bio-Rad repre-
sentative.
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