Bio-Rad Prepacked Gravity Flow Columns for Ion Exchange - Manual (Page 4)

Poly-Prep columns are ideal for routine
applications in which a small amount of chro-
matographic medium is used and then dis-
carded, or for multiple sample clean-up appli-
cations. They are a time-saving and versatile
replacement for Pasteur pipets and other
improvised columns.
Section 2
Column Description
The graduate 0.8 x 4 cm columns, con-
structed of high density polypropylene, hold a
standard bed volume of 2 ml of chromato-
graphic media, and include an integral 10 ml
reservoir. A Kynar
® 20 µm frit inside the col-
umn acts as a bed support for the resin pack-
ing. At the bottom of the column is a snap-off
seal, which leaves a male Luer tip fitting when
it is removed.
Prefilled Poly-Prep columns are shipped
sealed, to prevent the resin packing from dry-
ing. Columns are packed in deionized water.
During transport, the resin may move to the
top of the column. This is corrected by shaking
the column and allowing the resin to settle
before use. The columns should be stored
upright, in a dark, cool location.
Section 3
Instructions for Use
1. Shake the column and allow all the resin to
settle to the bottom.
2
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