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Bio-Rad Prepacked Gravity Flow Columns for Ion Exchange User Manual

Page 5

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2. Remove the column cap to allow the eluant

to flow freely.

3. Snap off the seal at the bottom of the

column.

4. Monitor the level of liquid in the reservoir,

and do not allow the resin to become dry.

5. Rinse the resin with several bed volumes

(at least 2 ml) of the sample solvent.
Discard rinse.

6. Add sample.

7. Collect effluent.

8. To reduce the flow rate or stop the flow,

use a Luer 3-way stopcock or the yellow
end-cap.

Section 4
Ion Exchange Capacity

The resin packing determines the ion

exchange capacity of each prefilled Poly-Prep
column. Table 1 lists the ion exchange capacity
of the resin in each prefilled Poly-Prep column.

Table 1. Ion Exchange Capacity of
Prefilled Poly-Prep Columns

Ion Exchange Capacity

Resin

(nominal meq/2 ml of rein)

AG

®

1-X8 resin

2.4

AG 2-X8 resin

2.4

AG 50W-X4 resin

2.2

AG 50W-X8 resin

3.4

Chelex

®

100 resin

0.8

Bio-Rex

®

70 resin

4.8

3

LIT84B 9/1/98 8:37 AM Page 3