Bio-Rad Prepacked Gravity Flow Columns for Ion Exchange - Manual (Page 8)

Section 7
Temperature Limits
Increases in temperature tend to decrease
resin selectivity, and can be used to decrease
elution times. Increased temperature also
increases exchange kinetics, resulting in
sharper resolution. However, some temperature
limits must be observed with ion exchange
resins. Table 3 gives the maximum tempera-
ture for each resin packing.
Table 3. Temperature Limits
Maximum
Resin Packing Temperature (°C)
AG 50 resin 150
AG 1 resin 150 (50 °C if converted
to OH
– form)
AG 2 resin 150° (30 °C if converted
to OH
– form)
Chelex 100 resin 75
Bio-Rex 70 resin 100
Section 8
Troubleshooting
1. Variation in color (lot-to-lot): Sometimes
the color of the resin may vary from lot to
lot. This will not affect the ion exchange
capacity of the resin.
2. Unusual odor: Amines will sometimes
cleave from anion exchange resin after pro-
longed storage. This is not unusual. They
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